Enzymatic Formation of Recombinant Antibody-Conjugated Gold Nanoparticles in the Presence of Citrate Groups and Bacteria

With the spread of deadly diseases worldwide, design rapid tests to identify causative microorganisms is necessary. Due unique properties gold nanoparticles, these nanoparticles are used in designing diagnostic tests, such as strip tests. The current study aimed investigate ability bind single-chain variable fragment antibodies. In this study, biological and chemical methods included Escherichia coli TOP-10 Turkevich method synthesize respectively. Then, effect synthetic on their capability binding recombinant antibodies was assessed by agarose gel UV-vis spectroscopy. Our result showed that had a spherical morphology, average size ~45 nm. Additionally, citrate groups were able serine residues antibody linker sequence; so, synthesis an effective strategy for can be promptly infectious microorganisms.